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CABRI:ICLC |
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Accession_number |
ICLC HTL96026
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Cell_line_name |
8305C
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Brief_description |
Species: human; Tissue: thyroid; Tumor: carcinoma, undifferentiated
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Description |
Species: human female 67 years old; Tissue: thyroid; Tumor: carcinoma, undifferentiated
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Depositor |
Obtained from ECACC.
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Bibliographic_reference |
-
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Morphology |
large spindle-shaped cells, grown as monolayer
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Culture_conditions |
continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 once a week using trypsin/EDTA. Doubling time is about 43 hours. Seed at 2-4 x 104 cells/cm2; cell harvest is about 2 x 106 cells/150 cm2 flask. 37C, 5% CO2.
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Viruses |
Search for viruses was not performed
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as human with AST, MD
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Biochem Biophys Res Commun 1994;198:948-954 [PMID: 8117301
]
Cancer Res 1992;52:1369 [PMID: 1737400
]
Int J Oncol 1994;4:583-586
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